How is detergent used in extracting dna




















Your teacher has already prepared a solution for you, made of onion treated with salt, distilled water and dishwashing detergent or shampoo. An onion is used because it has a low starch content, which allows the DNA to be seen clearly. The salt shields the negative phosphate ends of DNA, which allows the ends to come closer so the DNA can precipitate out of a cold alcohol solution. When you added the salt and detergent mixture, you probably mostly just saw more bubbles form in the bag because of the detergent.

After you added the cold rubbing alcohol to the filtered strawberry liquid, the alcohol should have precipitated the DNA out of the liquid while the rest of the liquid remained in solution. A single strand of DNA is extremely tiny, too tiny to see with the naked eye, but because the DNA clumped in this activity you were able to see just how much of it three strawberries have when all of their octoploid cells are combined!

This activity brought to you in partnership with Science Buddies. Already a subscriber? Sign in. Thanks for reading Scientific American. Create your free account or Sign in to continue. See Subscription Options. Discover World-Changing Science. Build a Cooler. The results show that the detergents could be used to extract genomic DNA.

Among the brands studied, five-enzyme Taj and three-enzyme Saftlan had the best performance compared to standard methods. AJOL and the millions of African and international researchers who rely on our free services are deeply grateful for your contribution.

Your donation is guaranteed to directly contribute to Africans sharing their research output with a global readership. Skip to main content Skip to main navigation menu Skip to site footer.



0コメント

  • 1000 / 1000